Citation:

journal.pone.0051590

Name: Interactive effects of ocean acidification and nitrogen limitation on the diatom Phaeodactylum tricornutum
Published: 2012
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Macro-nutrients; North Pacific; Ochrophyta; Phaeodactylum tricornutum; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species

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Abstract:

Climate change is expected to bring about alterations in the marine physical and chemical environment that will induce changes in the concentration of dissolved CO2 and in nutrient availability. These in turn are expected to affect the physiological performance of phytoplankton. In order to learn how phytoplankton respond to the predicted scenario of increased CO2 and decreased nitrogen in the surface mixed layer, we investigated the diatom Phaeodactylum tricornutum as a model organism. The cells were cultured in both low CO2 (390 µatm) and high CO2 (1000 µatm) conditions at limiting (10 µmol/L) or enriched (110 µmol/L) nitrate concentrations. Our study shows that nitrogen limitation resulted in significant decreases in cell size, pigmentation, growth rate and effective quantum yield of Phaeodactylum tricornutum, but these parameters were not affected by enhanced dissolved CO2 and lowered pH. However, increased CO2 concentration induced higher rETRmax and higher dark respiration rates and decreased the CO2 or dissolved inorganic carbon (DIC) affinity for electron transfer (shown by higher values for K1/2 DIC or K1/2 CO2). Furthermore, the elemental stoichiometry (carbon to nitrogen ratio) was raised under high CO2 conditions in both nitrogen limited and nitrogen replete conditions, with the ratio in the high CO2 and low nitrate grown cells being higher by 45% compared to that in the low CO2 and nitrate replete grown ones. Our results suggest that while nitrogen limitation had a greater effect than ocean acidification, the combined effects of both factors could act synergistically to affect marine diatoms and related biogeochemical cycles in future oceans.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Macro-nutrients; North Pacific; Ochrophyta; Phaeodactylum tricornutum; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-11-20.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Li, Wei
2	Identification	ID		Li, Wei
3	Treatment	Treat		Li, Wei		LC-HN=low pCO2 high NO3-, HC-HN=high CO2 high NO3-, LC-LN=low pCO2 low NO3-, LC-HN=low pCO2 high NO3-
4	Growth rate	µ	1/day	Li, Wei
5	Growth rate, standard deviation	µ std dev	±	Li, Wei
6	Chlorophyll a per cell	Chl a/cell	pg/#	Li, Wei	Spectrophotometric
7	Chlorophyll a, standard deviation	Chl a std dev	±	Li, Wei	Spectrophotometric
8	Carotenoids per cell	Carotenoids/cell	pg/#	Li, Wei	Spectrophotometric
9	Carotenoids, standard deviation	Carotenoids std dev	±	Li, Wei	Spectrophotometric
10	Chlorophyll c per cell	Chl c/cell	pg/#	Li, Wei	Spectrophotometric
11	Chlorophyll c, standard deviation	Chl c std dev	±	Li, Wei	Spectrophotometric
12	Cell size	Cell size	µm	Li, Wei
13	Cell counts, percent of total	Cell count	%	Li, Wei
14	Cell counts, standard deviation	Cell count std dev	±	Li, Wei
15	Maximum photochemical quantum yield of photosystem II	Fv/Fm		Li, Wei
16	Maximum photochemical quantum yield of photosystem II, standard deviation	Fv/Fm std dev	±	Li, Wei
17	Effective quantum yield	Y		Li, Wei
18	Effective quantum yield, standard deviation	Y std dev	±	Li, Wei
19	Cell size	Cell size	µm	Li, Wei
20	Cell size, standard deviation	Cell size std dev	±	Li, Wei
21	Cell biovolume	Cell biovol	µm³	Li, Wei
22	Cell biovolume, standard deviation	Cell biovol std dev	±	Li, Wei
23	Maximal electron transport rate, relative	rETR max		Li, Wei
24	Maximal electron transport rate, relative, standard deviation	rETR max std dev	±	Li, Wei
25	Carbon, inorganic, dissolved, reciprocal of photosynthetic affinity value	DIC K1/2	mmol/l	Li, Wei
26	Carbon, inorganic, dissolved, reciprocal of photosynthetic affinity value, standard deviation	DIC K1/2 std dev	±	Li, Wei
27	Carbon dioxide, reciprocal of photosynthetic affinity value	CO2 K1/2	µmol/l	Li, Wei
28	Carbon dioxide, reciprocal of photosynthetic affinity value, standard deviation	CO2 K1/2 std dev	±	Li, Wei
29	Carbon, inorganic, dissolved	DIC	µmol/kg	Li, Wei		values in figure 6
30	Photochemical efficiency	delta F/Fm	arbitrary units	Li, Wei		the apparent light use efficiency
31	Photochemical efficiency, standard deviation	delta F/Fm std dev	±	Li, Wei		the apparent light use efficiency
32	Respiration rate, oxygen, per cell	Resp O2/cell	fmol/#/h	Li, Wei
33	Respiration rate, oxygen, standard deviation	Resp O2 std dev	±	Li, Wei
34	Respiration rate, oxygen, per chlorophyll a	Resp O2/chl a	µmol/µg/h	Li, Wei
35	Respiration rate, oxygen, standard deviation	Resp O2 std dev	±	Li, Wei
36	Carbon, organic, particulate, per cell	POC/cell	pg/#	Li, Wei
37	Particulate organic carbon content per cell, standard deviation	POC cont/cell std dev	±	Li, Wei
38	Particulate organic nitrogen per cell	PON/cell	pg/#	Li, Wei
39	Particulate organic nitrogen per cell, standard deviation	PON/cell std dev	±	Li, Wei
40	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Li, Wei	Calculated using CO2SYS
41	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Li, Wei	Calculated using CO2SYS
42	pH	pH		Li, Wei	Potentiometric	NBS scale
43	pH, standard deviation	pH std dev	±	Li, Wei	Potentiometric	NBS scale
44	Carbon, inorganic, dissolved	DIC	µmol/kg	Li, Wei	Coulometric titration
45	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Li, Wei	Coulometric titration
46	Bicarbonate ion	[HCO3]-	µmol/kg	Li, Wei	Calculated using CO2SYS
47	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Li, Wei	Calculated using CO2SYS
48	Carbonate ion	[CO3]2-	µmol/kg	Li, Wei	Calculated using CO2SYS
49	Carbonate ion, standard deviation	[CO3]2- std dev	±	Li, Wei	Calculated using CO2SYS
50	Carbon dioxide	CO2	µmol/kg	Li, Wei	Calculated using CO2SYS
51	Carbon dioxide, standard deviation	CO2 std dev	±	Li, Wei	Calculated using CO2SYS
52	Alkalinity, total	AT	µmol/kg	Li, Wei	Calculated using CO2SYS
53	Alkalinity, total, standard deviation	AT std dev	±	Li, Wei	Calculated using CO2SYS
54	Temperature, water	Temp	°C	Li, Wei
55	Salinity	Sal		Li, Wei
56	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
57	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
58	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
59	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
60	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
61	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
62	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
63	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
64	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
65	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

29292 data points

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