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Li, Wei; Gao, Kunshan; Beardall, John (2012): Interactive effects of ocean acidification and nitrogen limitation on the diatom Phaeodactylum tricornutum [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.823110, Supplement to: Li, W et al. (2012): Interactive Effects of Ocean Acidification and Nitrogen-Limitation on the Diatom Phaeodactylum tricornutum. PLoS ONE, 7(12), e51590, https://doi.org/10.1371/journal.pone.0051590

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Abstract:
Climate change is expected to bring about alterations in the marine physical and chemical environment that will induce changes in the concentration of dissolved CO2 and in nutrient availability. These in turn are expected to affect the physiological performance of phytoplankton. In order to learn how phytoplankton respond to the predicted scenario of increased CO2 and decreased nitrogen in the surface mixed layer, we investigated the diatom Phaeodactylum tricornutum as a model organism. The cells were cultured in both low CO2 (390 µatm) and high CO2 (1000 µatm) conditions at limiting (10 µmol/L) or enriched (110 µmol/L) nitrate concentrations. Our study shows that nitrogen limitation resulted in significant decreases in cell size, pigmentation, growth rate and effective quantum yield of Phaeodactylum tricornutum, but these parameters were not affected by enhanced dissolved CO2 and lowered pH. However, increased CO2 concentration induced higher rETRmax and higher dark respiration rates and decreased the CO2 or dissolved inorganic carbon (DIC) affinity for electron transfer (shown by higher values for K1/2 DIC or K1/2 CO2). Furthermore, the elemental stoichiometry (carbon to nitrogen ratio) was raised under high CO2 conditions in both nitrogen limited and nitrogen replete conditions, with the ratio in the high CO2 and low nitrate grown cells being higher by 45% compared to that in the low CO2 and nitrate replete grown ones. Our results suggest that while nitrogen limitation had a greater effect than ocean acidification, the combined effects of both factors could act synergistically to affect marine diatoms and related biogeochemical cycles in future oceans.
Keyword(s):
Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Macro-nutrients; North Pacific; Ochrophyta; Phaeodactylum tricornutum; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species
Further details:
Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-11-20.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesLi, Wei
2IdentificationIDLi, Wei
3TreatmentTreatLi, WeiLC-HN=low pCO2 high NO3-, HC-HN=high CO2 high NO3-, LC-LN=low pCO2 low NO3-, LC-HN=low pCO2 high NO3-
4Growth rateµ1/dayLi, Wei
5Growth rate, standard deviationµ std dev±Li, Wei
6Chlorophyll a per cellChl a/cellpg/#Li, WeiSpectrophotometric
7Chlorophyll a, standard deviationChl a std dev±Li, WeiSpectrophotometric
8Carotenoids per cellCarotenoids/cellpg/#Li, WeiSpectrophotometric
9Carotenoids, standard deviationCarotenoids std dev±Li, WeiSpectrophotometric
10Chlorophyll c per cellChl c/cellpg/#Li, WeiSpectrophotometric
11Chlorophyll c, standard deviationChl c std dev±Li, WeiSpectrophotometric
12Cell sizeCell sizeµmLi, Wei
13Cell counts, percent of totalCell count%Li, Wei
14Cell counts, standard deviationCell count std dev±Li, Wei
15Maximum photochemical quantum yield of photosystem IIFv/FmLi, Wei
16Maximum photochemical quantum yield of photosystem II, standard deviationFv/Fm std dev±Li, Wei
17Effective quantum yieldYLi, Wei
18Effective quantum yield, standard deviationY std dev±Li, Wei
19Cell sizeCell sizeµmLi, Wei
20Cell size, standard deviationCell size std dev±Li, Wei
21Cell biovolumeCell biovolµm3Li, Wei
22Cell biovolume, standard deviationCell biovol std dev±Li, Wei
23Maximal electron transport rate, relativerETR maxLi, Wei
24Maximal electron transport rate, relative, standard deviationrETR max std dev±Li, Wei
25Carbon, inorganic, dissolved, reciprocal of photosynthetic affinity valueDIC K1/2mmol/lLi, Wei
26Carbon, inorganic, dissolved, reciprocal of photosynthetic affinity value, standard deviationDIC K1/2 std dev±Li, Wei
27Carbon dioxide, reciprocal of photosynthetic affinity valueCO2 K1/2µmol/lLi, Wei
28Carbon dioxide, reciprocal of photosynthetic affinity value, standard deviationCO2 K1/2 std dev±Li, Wei
29Carbon, inorganic, dissolvedDICµmol/kgLi, Weivalues in figure 6
30Photochemical efficiencydelta F/Fmarbitrary unitsLi, Weithe apparent light use efficiency
31Photochemical efficiency, standard deviationdelta F/Fm std dev±Li, Weithe apparent light use efficiency
32Respiration rate, oxygen, per cellResp O2/cellfmol/#/hLi, Wei
33Respiration rate, oxygen, standard deviationResp O2 std dev±Li, Wei
34Respiration rate, oxygen, per chlorophyll aResp O2/chl aµmol/µg/hLi, Wei
35Respiration rate, oxygen, standard deviationResp O2 std dev±Li, Wei
36Carbon, organic, particulate, per cellPOC/cellpg/#Li, Wei
37Particulate organic carbon content per cell, standard deviationPOC cont/cell std dev±Li, Wei
38Particulate organic nitrogen per cellPON/cellpg/#Li, Wei
39Particulate organic nitrogen per cell, standard deviationPON/cell std dev±Li, Wei
40Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmLi, WeiCalculated using CO2SYS
41Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Li, WeiCalculated using CO2SYS
42pHpHLi, WeiPotentiometricNBS scale
43pH, standard deviationpH std dev±Li, WeiPotentiometricNBS scale
44Carbon, inorganic, dissolvedDICµmol/kgLi, WeiCoulometric titration
45Carbon, inorganic, dissolved, standard deviationDIC std dev±Li, WeiCoulometric titration
46Bicarbonate ion[HCO3]-µmol/kgLi, WeiCalculated using CO2SYS
47Bicarbonate ion, standard deviation[HCO3]- std dev±Li, WeiCalculated using CO2SYS
48Carbonate ion[CO3]2-µmol/kgLi, WeiCalculated using CO2SYS
49Carbonate ion, standard deviation[CO3]2- std dev±Li, WeiCalculated using CO2SYS
50Carbon dioxideCO2µmol/kgLi, WeiCalculated using CO2SYS
51Carbon dioxide, standard deviationCO2 std dev±Li, WeiCalculated using CO2SYS
52Alkalinity, totalATµmol/kgLi, WeiCalculated using CO2SYS
53Alkalinity, total, standard deviationAT std dev±Li, WeiCalculated using CO2SYS
54Temperature, waterTemp°CLi, Wei
55SalinitySalLi, Wei
56Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
57pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
58Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
59Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
60Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
61Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
62Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
63Alkalinity, totalATµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
64Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
65Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
29292 data points

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